Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer

Tetsushi Taguchi, Liming Xu, Hisatoshi Kobayashi, Akiyoshi Taniguchi, Kazunori Kataoka, Junzo Tanaka

研究成果: Article査読

73 被引用数 (Scopus)

抄録

An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0mM. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type II collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects.

本文言語English
ページ(範囲)1247-1252
ページ数6
ジャーナルBiomaterials
26
11
DOI
出版ステータスPublished - 2005 4
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • セラミックおよび複合材料
  • 生物理学
  • 生体材料
  • 材料力学

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