Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Hiromi Sakai, Shinji Takeoka, Hiroaki Yokohama, Hiroyuki Nishide, Eishun Tsuchida

研究成果: Article

15 引用 (Scopus)

抜粋

A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

元の言語English
ページ(範囲)389-394
ページ数6
ジャーナルPolymers for Advanced Technologies
3
発行部数7
DOI
出版物ステータスPublished - 1992

    フィンガープリント

ASJC Scopus subject areas

  • Polymers and Plastics

これを引用