Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor

Yoshinori Abe, Yoshiyuki Ozaki, Junichi Kasuya, Kimiko Yamamoto, Joji Ando, Ryo Sudo, Mariko Ikeda, Kazuo Tanishita

研究成果: Article

14 引用 (Scopus)

抄録

Endothelial progenitor cell (EPC) transplantation induces the formation of new blood-vessel networks to supply nutrients and oxygen, and is feasible for the treatment of ischemia and cardiovascular diseases. However, the role of EPCs as a source of proangiogenic cytokines and consequent generators of an extracellular growth factor microenvironment in three-dimensional (3D) microvessel formation is not fully understood. We focused on the contribution of EPCs as a source of proangiogenic cytokines on 3D microvessel formation using an in vitro 3D network model. To create a 3D network model, EPCs isolated from rat bone marrow were sandwiched with double layers of collagen gel. Endothelial cells (ECs) were then cultured on top of the upper collagen gel layer. Quantitative analyses of EC network formation revealed that the length, number, and depth of the EC networks were significantly enhanced in a 3D model with ECs and EPCs compared to an EC monoculture. In addition, conditioned medium (CM) from the 3D model with ECs and EPCs promoted network formation compared to CM from an EC monoculture. We also confirmed that EPCs secreted vascular endothelial growth factor (VEGF). However, networks cultured with the CM were shallow and did not penetrate the collagen gel in great depth. Therefore, we conclude that EPCs contribute to 3D network formation at least through indirect incorporation by generating a local VEGF gradient. These results suggest that the location of EPCs is important for controlling directional 3D network formation in the field of tissue engineering.

元の言語English
記事番号e82085
ジャーナルPLoS One
8
発行部数12
DOI
出版物ステータスPublished - 2013 12 3

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vascular endothelial growth factors
Endothelial cells
Vascular Endothelial Growth Factor A
endothelial cells
stem cells
Endothelial Cells
Conditioned Culture Medium
collagen
Collagen
Gels
gels
Microvessels
cytokines
Cytokines
tissue engineering
cell transplantation
Endothelial Progenitor Cells
erucylphosphocholine
generators (equipment)
Cell Transplantation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

これを引用

Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. / Abe, Yoshinori; Ozaki, Yoshiyuki; Kasuya, Junichi; Yamamoto, Kimiko; Ando, Joji; Sudo, Ryo; Ikeda, Mariko; Tanishita, Kazuo.

:: PLoS One, 巻 8, 番号 12, e82085, 03.12.2013.

研究成果: Article

Abe, Yoshinori ; Ozaki, Yoshiyuki ; Kasuya, Junichi ; Yamamoto, Kimiko ; Ando, Joji ; Sudo, Ryo ; Ikeda, Mariko ; Tanishita, Kazuo. / Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. :: PLoS One. 2013 ; 巻 8, 番号 12.
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