抄録
Definition of rhodopsin is the retinal-binding membrane protein with the Schiff base linkage at a lysine on the 7th transmembrane helix. However, ~ 600 microbial rhodopsins lack retinal-binding lysine at the corresponding position (Rh-noK) among ~ 5500 known microbial rhodopsins, suggesting that Rh-noK has each functional role without chromophore. Here, we report successful functional recovery of Rh-noK. Two Rh-noKs from bacteria were heterologously expressed in Escherichia coli, which exhibited no color. When retinal-binding lysine was introduced, one of them gained visible color. Additional mutation of the Schiff base counterion further gained proton-pumping activity. Successful engineered functional recovery such as visible color and proton-pump activity suggests that the Rh-noK protein forms a characteristic structure of microbial rhodopsins.
本文言語 | English |
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ページ(範囲) | 1116-1121 |
ページ数 | 6 |
ジャーナル | Photochemistry and Photobiology |
巻 | 95 |
号 | 5 |
DOI | |
出版ステータス | Published - 2019 9月 1 |
ASJC Scopus subject areas
- 放射線
- 生化学
- 物理化学および理論化学