Engineered Functional Recovery of Microbial Rhodopsin Without Retinal-Binding Lysine

Yumeka Yamauchi, Masae Konno, Daichi Yamada, Kei Yura, Keiichi Inoue, Oded Béjà, Hideki Kandori*

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Definition of rhodopsin is the retinal-binding membrane protein with the Schiff base linkage at a lysine on the 7th transmembrane helix. However, ~ 600 microbial rhodopsins lack retinal-binding lysine at the corresponding position (Rh-noK) among ~ 5500 known microbial rhodopsins, suggesting that Rh-noK has each functional role without chromophore. Here, we report successful functional recovery of Rh-noK. Two Rh-noKs from bacteria were heterologously expressed in Escherichia coli, which exhibited no color. When retinal-binding lysine was introduced, one of them gained visible color. Additional mutation of the Schiff base counterion further gained proton-pumping activity. Successful engineered functional recovery such as visible color and proton-pump activity suggests that the Rh-noK protein forms a characteristic structure of microbial rhodopsins.

本文言語English
ページ(範囲)1116-1121
ページ数6
ジャーナルPhotochemistry and Photobiology
95
5
DOI
出版ステータスPublished - 2019 9 1

ASJC Scopus subject areas

  • 放射線
  • 生化学
  • 物理化学および理論化学

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