Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins

Fuun Kawano, Hideyuki Suzuki, Akihiro Furuya, Moritoshi Sato*

*この研究の対応する著者

研究成果査読

188 被引用数 (Scopus)

抄録

Optogenetic methods take advantage of photoswitches to control the activity of cellular proteins. Here, we completed a multi-directional engineering of the fungal photoreceptor Vivid to develop pairs of distinct photoswitches named Magnets. These new photoswitches were engineered to recognize each other based on the electrostatic interactions, thus preventing homodimerization and enhancing light-induced heterodimerization. Furthermore, we tuned the switch-off kinetics by four orders of magnitude and developed several variants, including those with substantially faster kinetics than any of the other conventional dimerization-based blue spectrum photoswitches. We demonstrate the utility of Magnets as powerful tools that can optogenetically manipulate molecular processes in biological systems.

本文言語English
論文番号6256
ジャーナルNature Communications
6
DOI
出版ステータスPublished - 2015 2 25
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 化学 (全般)
  • 物理学および天文学(全般)

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