Enzymatic replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr and its effect on aminoacylation.

T. Ohyama*, K. Nishikawa, S. Takemura

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

An efficient method for replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr has been developed. An abnormal tRNATyr lacking in tetranucleotide D16-D-Gm-G19 in its D-loop has been reconstructed by this method and shown to accept tyrosine to about 55% of the aminoacylation level observed for intact tRNATyr. This suggests that the deleted sequence itself is not essential for recognition by TyrRS but a conformational instability of the tRNA possibly caused by the disruption of tertiary interactions between the D-loop and T psi C-loop might have influenced the forward reaction rate leading to the decreased level of aminoacylation.

本文言語English
ページ(範囲)137-140
ページ数4
ジャーナルNucleic acids symposium series
12
出版ステータスPublished - 1983 12 1
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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