抜粋
Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 33-37 |
| ページ数 | 5 |
| ジャーナル | Journal of Biochemistry |
| 巻 | 80 |
| 発行部数 | 1 |
| 出版物ステータス | Published - 1976 7 |
| 外部発表 | Yes |
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Molecular Biology
- Biochemistry
フィンガープリント Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。
これを引用
Kikuchi, Y., Hirai, K., Someno, K., & Sakaguchi, K. (1976). Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase. Journal of Biochemistry, 80(1), 33-37.