Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase

Yo Kikuchi, Keiko Hirai, Kinumi Someno, Kenji Sakaguchi

研究成果: Article › 査読

6 被引用数 (Scopus)

抄録

Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.

本文言語English
ページ（範囲）33-37
ページ数5
ジャーナルJournal of Biochemistry
巻80
号1
出版ステータスPublished - 1976 7
外部発表はい

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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