抄録
Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 33-37 |
| ページ数 | 5 |
| ジャーナル | Journal of Biochemistry |
| 巻 | 80 |
| 発行部数 | 1 |
| 出版物ステータス | Published - 1976 7 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Molecular Biology
- Biochemistry
これを引用
Enzymatic synthesis of oligonucleotides of defined sequence : The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase. / Kikuchi, Yo; Hirai, Keiko; Someno, Kinumi; Sakaguchi, Kenji.
：: Journal of Biochemistry, 巻 80, 番号 1, 07.1976, p. 33-37.研究成果: Article
}
TY - JOUR
T1 - Enzymatic synthesis of oligonucleotides of defined sequence
T2 - The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase
AU - Kikuchi, Yo
AU - Hirai, Keiko
AU - Someno, Kinumi
AU - Sakaguchi, Kenji
PY - 1976/7
Y1 - 1976/7
N2 - Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.
AB - Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.
UR - http://www.scopus.com/inward/record.url?scp=0017169246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017169246&partnerID=8YFLogxK
M3 - Article
C2 - 9379
AN - SCOPUS:0017169246
VL - 80
SP - 33
EP - 37
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 1
ER -