Epigenetic characterization of the CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses.

Maksym Kremenskoy*, Yuliya Kremenska, Masako Suzuki, Kei Imai, Seiya Takahashi, Kazuyoshi Hashizume, Shintaro Yagi, Kunio Shiota

*この研究の対応する著者

研究成果: Article査読

22 被引用数 (Scopus)

抄録

Abnormal development and fetal loss during postimplantation period are concerns for production of nuclear transferred animals. Aberrant DNA methylation is one of the reasons for poor survival of cloned animals. In mammalian genome DNA, CpG islands are preferentially located at the start of transcription of housekeeping genes and are associated with tissue-specific genes. The correct and consecutive mechanisms of DNA methylation in the CpG islands are necessary for selective gene expressions that determine the properties of individual cells, tissues, and organs. In this study, we investigated the methylation status of the CpG islands of the bovine Leptin and POU5F1 genes in fetal and placental tissues from fetuses produced by artificial insemination (AI) and nuclear transfer (NT) at days 48 and 59 of pregnancy. Altered DNA methylation was observed in the normal and cloned fetal, placental, and endometrial tissues using bisulfite sequencing and pyrosequencing. Different tissue-specific methylated regions in the bovine Leptin and POU5F1 genes show a variable methylation status in NT fetuses compared to AI control.

本文言語English
ページ(範囲)277-285
ページ数9
ジャーナルJournal of Reproduction and Development
52
2
DOI
出版ステータスPublished - 2006 4月
外部発表はい

ASJC Scopus subject areas

  • 動物科学および動物学
  • 生殖医学

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