Epigenetic effects of insecticides on early differentiation of mouse embryonic stem cells

Wenlong Wang, Tomohiro Ito, Satoshi Otsuka, Hiroko Nansai, Kuniya Abe, Yoichi Nakao, Jun Ohgane, Minoru Yoneda, Hideko Sone

研究成果: Article査読

抄録

Increasing evidence indicates that many insecticides produce significant epigenetic changes during embryogenesis, leading to developmental toxicities. However, the effects of insecticides on DNA methylation status during early development have not been well studied. We developed a novel nuclear phenotypic approach using mouse embryonic stem cells harboring enhanced green fluorescent protein fused with methyl CpG-binding protein to evaluate global DNA methylation changes via high-content imaging analysis. Exposure to imidacloprid, carbaryl, and o,p'-DDT increased the fluorescent intensity of granules in the nuclei, indicating global DNA methylating effects. However, DNA methylation profiling in cell-cycle-related genes, such as Cdkn2a, Dapk1, Cdh1, Mlh1, Timp3, and Rarb, decreased in imidacloprid treatments, suggesting the potential influence of DNA methylation patterns on cell differentiation. We developed a rapid method for evaluating global DNA methylation and used this approach to show that insecticides pose risks of developmental toxicity through DNA methylation.

本文言語English
論文番号105174
ジャーナルToxicology in Vitro
75
DOI
出版ステータスPublished - 2021 9

ASJC Scopus subject areas

  • 毒物学

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