Epigenetic inheritance of elongated phenotypes between generations revealed by individual-cell-based direct observation

Yuichi Wakamoto, Kenji Yasuda

研究成果: Article

5 引用 (Scopus)

抄録

A cellular phenotype is considered to be determined not only by genetic information but also by convoluted information on past states of a cell and its ancestors, i.e. hysteresis. This 'hysteretic effect' forms the basis of epigenetic phenomena. To understand these phenomena by which cells transmit certain phenotypes to descendants, it is necessary to observe individual cells and compare the phenotypes of each between generations under stringently controlled environmental conditions. We, therefore, did an individual-cell-based differential assay using Escherichia coli as a model organism. We observed normal-sized isolated cells change into elongated phenotypes, and subsequently measured the transmission of their characteristics between generations. This change occurred when the final length of the normal cells exceeded their cell-length boundary, i.e., 10 νm with 5% probability. Once a cell became elongated, it divided unequally, producing two daughter cells; one was elongated and the other was normal. The elongated daughter transmitted the elongated phenotype to one lineage of the descendants by repeating unequal cell divisions with an average interdivision time half that of the normal phenotype, whereas the normal daughter retained normal phenotypic characteristics. The results suggest one possible non-genetic inheritance of cellular characteristics where phenotypic differences can only be inherited by geometrical information, independent of specific gene regulation.

元の言語English
記事番号S09
ページ(範囲)3171-3177
ページ数7
ジャーナルMeasurement Science and Technology
17
発行部数12
DOI
出版物ステータスPublished - 2006 12 1
外部発表Yes

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phenotype
Gene expression
Phenotype
Escherichia coli
Hysteresis
Assays
Cells
Cell
cells
cell division
Observation
Gene Regulation
gene expression
Cell Division
Time-average
Escherichia
Unequal
organisms
Escherichia Coli
hysteresis

ASJC Scopus subject areas

  • Polymers and Plastics
  • Ceramics and Composites
  • Materials Science (miscellaneous)

これを引用

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abstract = "A cellular phenotype is considered to be determined not only by genetic information but also by convoluted information on past states of a cell and its ancestors, i.e. hysteresis. This 'hysteretic effect' forms the basis of epigenetic phenomena. To understand these phenomena by which cells transmit certain phenotypes to descendants, it is necessary to observe individual cells and compare the phenotypes of each between generations under stringently controlled environmental conditions. We, therefore, did an individual-cell-based differential assay using Escherichia coli as a model organism. We observed normal-sized isolated cells change into elongated phenotypes, and subsequently measured the transmission of their characteristics between generations. This change occurred when the final length of the normal cells exceeded their cell-length boundary, i.e., 10 νm with 5{\%} probability. Once a cell became elongated, it divided unequally, producing two daughter cells; one was elongated and the other was normal. The elongated daughter transmitted the elongated phenotype to one lineage of the descendants by repeating unequal cell divisions with an average interdivision time half that of the normal phenotype, whereas the normal daughter retained normal phenotypic characteristics. The results suggest one possible non-genetic inheritance of cellular characteristics where phenotypic differences can only be inherited by geometrical information, independent of specific gene regulation.",
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AB - A cellular phenotype is considered to be determined not only by genetic information but also by convoluted information on past states of a cell and its ancestors, i.e. hysteresis. This 'hysteretic effect' forms the basis of epigenetic phenomena. To understand these phenomena by which cells transmit certain phenotypes to descendants, it is necessary to observe individual cells and compare the phenotypes of each between generations under stringently controlled environmental conditions. We, therefore, did an individual-cell-based differential assay using Escherichia coli as a model organism. We observed normal-sized isolated cells change into elongated phenotypes, and subsequently measured the transmission of their characteristics between generations. This change occurred when the final length of the normal cells exceeded their cell-length boundary, i.e., 10 νm with 5% probability. Once a cell became elongated, it divided unequally, producing two daughter cells; one was elongated and the other was normal. The elongated daughter transmitted the elongated phenotype to one lineage of the descendants by repeating unequal cell divisions with an average interdivision time half that of the normal phenotype, whereas the normal daughter retained normal phenotypic characteristics. The results suggest one possible non-genetic inheritance of cellular characteristics where phenotypic differences can only be inherited by geometrical information, independent of specific gene regulation.

KW - Elongated phenotypes

KW - Epigenetic inheritance

KW - Escherichia coli

KW - Hysteresis

KW - Individual-cell-based direct observation

KW - Microchamber

KW - Non-genetic inheritance

KW - Optical trapping

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