Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice

Kunio Shiota*, Yasushi Kogo, Jun Ohgane, Takuya Imamura, Atsushi Urano, Koichiro Nishino, Satoshi Tanaka, Naka Hattori

*この研究の対応する著者

研究成果: Article査読

174 被引用数 (Scopus)

抄録

Background: DNA methylation is involved in many gene functions such as gene-silencing, X-inactivation, imprinting and stability of the gene. We recently found that some CpG islands had a tissue-dependent and differentially methylated region (T-DMR) in normal tissues, raising the possibility that there may be more CpG islands capable of differential methylation. Results: We investigated the genome-wide DNA methylation pattern of CpG islands by restriction landmark genomic scanning (RLGS) in mouse stem cells (ES, EG and trophoblast stem) before and after differentiation, and sperm as well as somatic tissues. A total of 247 spots out of 1500 (16%) showed differences in the appearance of their RLGS profiles, indicating that CpG islands having T-DMR were numerous and widespread. The methylation pattern was specific, and varied in a precise manner according to cell lineage, tissue type and during cell differentiation. Conclusions: Genomic loci with altered methylation status seem to be more common than has hitherto been realized. The formation of DNA methylation patterns at CpG islands is one of the epigenetic events which underlies the production of various cell types in the body. These findings should have implications for the use of embryonic stem cells and cells derived from them therapeutically, and also for the cloning of animals by the transfer of somatic cell nuclei.

本文言語English
ページ(範囲)961-969
ページ数9
ジャーナルGenes to Cells
7
9
DOI
出版ステータスPublished - 2002 9月
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 細胞生物学

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