Epigenetic regulation of Nanog gene in embryonic stem and trophoblast stem cells

Naoko Hattori, Yuko Imao, Koichiro Nishino, Naka Hattori, Jun Ohgane, Shintaro Yagi, Satoshi Tanaka, Kunio Shiota

研究成果: Article査読

147 被引用数 (Scopus)

抄録

The Nanog and Oct-4 genes are essential for maintaining pluripotency of embryonic stem (ES) cells and early embryos. We previously reported that DNA methylation and chromatin remodeling underlie the cell type-specific mechanism of Oct-4 gene expression. In the present study, we found that there is a tissue-dependent and differentially methylated region (T-DMR) in the Nanog up-stream region. The T-DMR is hypomethylated in ES cells, but is heavily methylated in trophoblast stem (TS) cells and NIH/3T3 cells, in which the Nanog gene is repressed. Furthermore, in vitro methylation of T-DMR suppressed Nanog promoter activity in reporter assay. Chromatin immunoprecipitation assay revealed that histone H3 and H4 are highly acetylated, and H3 lysine (K) 4 is hypermethylated at the Nanog locus in ES cells. Conversely, histone deacetylation and H3-K4 demethylation occurred in TS cells. Importantly, in TS cells, hypermethylation of H3-K9 and -K27 is found only at the Nanog locus, not the Oct-4 locus, indicating that the combination of histone modifications associated with the Nanog gene is distinct from that of the Oct-4 gene. In conclusion, the Nanog gene is regulated by epigenetic mechanisms involving DNA methylation and histone modifications.

本文言語English
ページ(範囲)387-396
ページ数10
ジャーナルGenes to Cells
12
3
DOI
出版ステータスPublished - 2007 3
外部発表はい

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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