Estrogen receptors in the stingray (Dasyatis akajei) ultimobranchial gland

Kazutoshi Yamamoto*, Nobuo Suzuki, Noriyuki Takahashi, Yuichi Sasayama, Sakae Kikuyama

*この研究の対応する著者

    研究成果: Article査読

    13 被引用数 (Scopus)

    抄録

    On the basis of our previous observation that estrogen stimulates calcitonin secretion by the ultimobranchial gland in the stingray, Dasyatis akajei, experiments were conducted to examine the presence of estrogen receptor (ER) and its mRNA in the gland, employing exchange assay and Northern blot analysis, respectively. The optimal incubation conditions of the exchange assay for cytosolic ER were found to be 25° for 2 hr. Scatchard analysis of cytosolic ER revealed two components with dissociation constants (K(d)) of 0.090 ± 0.015 and 17.780 ± 1.910 nM and with a maximum number of binding sites (B(max)) of 62.2 ± 7.9 and 2290 ± 180 fmol/mg protein, respectively. Furthermore, Sephadex G-100 filtration of the cytosol and immunoblot analysis using antiserum against a recombinant rat ER confirmed the presence of two specific binding components for estrogen (41 and 57 kDa). Total RNA extracted from the ultimobranchial gland was subjected to Northern blot analysis using a rat ER cDNA as a probe. Two positive signals were detected at 2.5 and 2.0 kb. Thus, the presence of ER was confirmed for the first time in nonmammalian calcitonin-secreting cells.

    本文言語English
    ページ(範囲)107-114
    ページ数8
    ジャーナルGeneral and Comparative Endocrinology
    101
    1
    DOI
    出版ステータスPublished - 1996 1

    ASJC Scopus subject areas

    • 内分泌学

    フィンガープリント

    「Estrogen receptors in the stingray (Dasyatis akajei) ultimobranchial gland」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル