Evaluation of imaging biomarkers for identification of single cancer cells in blood

Masao Odaka, Hyonchol Kim, Mathias Girault, Akihiro Hattori, Hideyuki Terazono, Kenji Matsuura, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

抄録

A method of discriminating single cancer cells from whole blood cells based on their morphological visual characteristics (i.e., "imaging biomarker") was examined. Cells in healthy rat blood, a cancer cell line (MAT-LyLu), and cells in cancer-cell-implanted rat blood were chosen as models, and their bright-field (BF, whole-cell morphology) and fluorescence (FL, nucleus morphology) images were taken by an on-chip multi-imaging flow cytometry system and compared. Eight imaging biomarker indices, i.e., cellular area in a BF image, nucleus area in an FL image, area ratio of a whole cell and its nucleus, distance of the mass center between a whole cell and nucleus, cellular and nucleus perimeter, and perimeter ratios were calculated and analyzed using the BF and FL images taken. Results show that cancer cells can be clearly distinguished from healthy blood cells using correlation diagrams for cellular and nucleus areas as two different categories. Moreover, a portion of cancer cells showed a low nucleus perimeter ratio less than 0.9 because of the irregular nucleus morphologies of cancer cells. These results indicate that the measurements of imaging biomarkers are practically applicable to identifying cancer cells in blood.

本文言語English
論文番号06FN04
ジャーナルJapanese journal of applied physics
54
6
DOI
出版ステータスPublished - 2015 6月 1
外部発表はい

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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