Light-emitting diodes (LEDs) are widely used for backlighting text indicators in vehicles. It is desirable that such text indicators have high legibility and low fatigue-inducing qualities. Suitable spectral distributions for text presentation in vehicles have not yet been clarified. This study examined legibility and fatigue when participants read Japanese sentences presented through backlight emission for 10, min in a dark room. The backlight indicators employed four type of white LEDs. The participants were asked to answer questionnaires to evaluate legibility and fatigue. Moreover, the critical fusion frequency and brain activity were measured to evaluate fatigue objectively. The questionnaire results show that all colored backlighting displayed almost an equal legibility. The overall result suggests that participants were most and least fatigued when viewing text illuminated by high color-rending and greenish white backlighting, respectively. Thus, fatigue can be reduced using LEDs with different spectral distributions.