Evolution of A Pathway to Novel Long-Chain Carotenoids

Daisuke Umeno*, Frances H. Arnold

*この研究の対応する著者

研究成果: Article査読

61 被引用数 (Scopus)

抄録

Using methods of laboratory evolution to force the C30 carotenoid synthase CrtM to function as a C40 synthase, followed by further mutagenesis at functionally important amino acid residues, we have discovered that synthase specificity is controlled at the second (rearrangement) step of the two-step reaction. We used this information to engineer CrtM variants that can synthesize previously unknown C45 and C 50 carotenoid backbones (mono- and diisopentenylphytoenes) from the appropriate isoprenyldiphosphate precursors. With this ability to produce new backbones in Escherichia coli comes the potential to generate whole series of novel carotenoids by using carotenoid-modifying enzymes, including desaturases, cyclases, hydroxylases, and dioxygenases, from naturally occurring pathways.

本文言語English
ページ(範囲)1531-1536
ページ数6
ジャーナルJournal of Bacteriology
186
5
DOI
出版ステータスPublished - 2004 3
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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