Evolutionary origin of GnIH and NPFF in chordates: Insights from novel amphioxus RFamide peptides

Tomohiro Osugi, Tomoki Okamura, You Lee Son, Makoto Ohkubo, Takayoshi Ubuka, Yasuhisa Henmi, Kazuyoshi Tsutsui

    研究成果: Article査読

    27 被引用数 (Scopus)

    抄録

    Gonadotropin-inhibitory hormone (GnIH) is a newly identified hypothalamic neuropeptide that inhibits pituitary hormone secretion in vertebrates. GnIH has an LPXRFamide (X = L or Q) motif at the C-terminal in representative species of gnathostomes. On the other hand, neuropeptide FF (NPFF), a neuropeptide characterized as a pain-modulatory neuropeptide, in vertebrates has a PQRFamide motif similar to the C-terminal of GnIH, suggesting that GnIH and NPFF have diverged from a common ancestor. Because GnIH and NPFF belong to the RFamide peptide family in vertebrates, protochordate RFamide peptides may provide important insights into the evolutionary origin of GnIH and NPFF. In this study, we identified a novel gene encoding RFamide peptides and two genes of their putative receptors in the amphioxus Branchiostoma japonicum. Molecular phylogenetic analysis and synteny analysis indicated that these genes are closely related to the genes of GnIH and NPFF and their receptors of vertebrates. We further identified mature RFamide peptides and their receptors in protochordates. The identified amphioxus RFamide peptides inhibited forskolin induced cAMP signaling in the COS-7 cells with one of the identified amphioxus RFamide peptide receptors expressed. These results indicate that the identified protochordate RFamide peptide gene is a common ancestral form of GnIH and NPFF genes, suggesting that the origin of GnIH and NPFF may date back to the time of the emergence of early chordates. GnIH gene and NPFF gene may have diverged by whole-genome duplication in the course of vertebrate evolution.

    本文言語English
    論文番号e100962
    ジャーナルPLoS One
    9
    7
    DOI
    出版ステータスPublished - 2014 7月 1

    ASJC Scopus subject areas

    • 農業および生物科学(全般)
    • 生化学、遺伝学、分子生物学(全般)
    • 医学(全般)

    フィンガープリント

    「Evolutionary origin of GnIH and NPFF in chordates: Insights from novel amphioxus RFamide peptides」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル