Excited-state dynamics of bacteriorhodopsin.

T. Kouyama, K. Kinosita, A. Ikegami

    研究成果: Article査読

    31 被引用数 (Scopus)


    Near infrared emission of bacteriorhodopsin at neutral pH and at room temperature was characterized by a large Stokes shift. This characteristic was lost in an acidic pH (approximately pH 2) where a remarkable enchancement (more than 10 times) in the fluorescence quantum yield accompanied the red shift in the main absorption band. It is suggested from fluorescence polarization measurements that the emission occurs from the first allowed excited state of the retinylidene chromophore, irrespective of pH. We suggest that the large Stokes shift observed at neutral pH is a result of a charge displacement (e.g., proton translocation) that occurs immediately after excitation, and is prevented by protonation (in the ground state) of an amino-acid residue in the protein.

    ジャーナルBiophysical Journal
    出版ステータスPublished - 1985 1

    ASJC Scopus subject areas

    • Biophysics

    フィンガープリント 「Excited-state dynamics of bacteriorhodopsin.」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。