Excited-state dynamics of bacteriorhodopsin.

T. Kouyama, K. Kinosita, A. Ikegami

    研究成果: Article

    30 引用 (Scopus)

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    Near infrared emission of bacteriorhodopsin at neutral pH and at room temperature was characterized by a large Stokes shift. This characteristic was lost in an acidic pH (approximately pH 2) where a remarkable enchancement (more than 10 times) in the fluorescence quantum yield accompanied the red shift in the main absorption band. It is suggested from fluorescence polarization measurements that the emission occurs from the first allowed excited state of the retinylidene chromophore, irrespective of pH. We suggest that the large Stokes shift observed at neutral pH is a result of a charge displacement (e.g., proton translocation) that occurs immediately after excitation, and is prevented by protonation (in the ground state) of an amino-acid residue in the protein.

    元の言語English
    ページ(範囲)43-54
    ページ数12
    ジャーナルBiophysical Journal
    47
    発行部数1
    出版物ステータスPublished - 1985 1

      フィンガープリント

    ASJC Scopus subject areas

    • Biophysics

    これを引用

    Kouyama, T., Kinosita, K., & Ikegami, A. (1985). Excited-state dynamics of bacteriorhodopsin. Biophysical Journal, 47(1), 43-54.