抄録
Nonalcoholic steatohepatitis, which is considered the hepatic event in metabolic syndrome, was recently associated with the innate immune system. Although regular exercise reduces hepatic injury markers like serum alanine aminotransferase (ALT) levels, the mechanisms regulating the effects of exercise on steatohepatitis are unclear. This study aimed to clarify whether exercise training suppresses hepatic injury, inflammation, and fibrosis by suppressing macrophage infiltration. Male C57BL/6J (4-week old) mice were randomly divided into four groups: normal diet (ND) control (. n=. 7), ND exercise (. n=. 5), high-fat diet and high-fructose water (HFF) control (. n=. 11), and HFF exercise (. n=. 11) groups. Mice were fed the ND or HFF from 4 to 20. weeks of age. The exercise groups were trained on a motorized treadmill for 60. min/day, five times/week. The nonalcoholic fatty liver disease (NAFLD) activity score and plasma ALT activity, indicators of liver injury, were increased in HFF control mice but were attenuated in HFF exercise mice. Hepatic inflammation, indicated by hepatic tumor necrosis factor (TNF)-α levels and hepatic resident macrophage infiltration, was significantly lower in HFF exercise mice than in HFF control mice. Hepatic fibrosis markers (histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining and tissue inhibitor of matrix metalloproteinase-1 mRNA) were attenuated in HFF exercise mice compared with HFF control mice. These results suggest that exercise training reduces hepatic inflammation, injury, and fibrosis by suppressing macrophage infiltration.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 931-941 |
| ページ数 | 11 |
| ジャーナル | Brain, Behavior, and Immunity |
| 巻 | 26 |
| 発行部数 | 6 |
| DOI | |
| 出版物ステータス | Published - 2012 8 |
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ASJC Scopus subject areas
- Immunology
- Behavioral Neuroscience
- Endocrine and Autonomic Systems
これを引用
Exercise training attenuates hepatic inflammation, fibrosis and macrophage infiltration during diet induced-obesity in mice. / Kawanishi, Noriaki; Yano, Hiromi; Mizokami, Tsubasa; Takahashi, Masaki; Oyanagi, Eri; Suzuki, Katsuhiko.
：: Brain, Behavior, and Immunity, 巻 26, 番号 6, 08.2012, p. 931-941.研究成果: Article
}
TY - JOUR
T1 - Exercise training attenuates hepatic inflammation, fibrosis and macrophage infiltration during diet induced-obesity in mice
AU - Kawanishi, Noriaki
AU - Yano, Hiromi
AU - Mizokami, Tsubasa
AU - Takahashi, Masaki
AU - Oyanagi, Eri
AU - Suzuki, Katsuhiko
PY - 2012/8
Y1 - 2012/8
N2 - Nonalcoholic steatohepatitis, which is considered the hepatic event in metabolic syndrome, was recently associated with the innate immune system. Although regular exercise reduces hepatic injury markers like serum alanine aminotransferase (ALT) levels, the mechanisms regulating the effects of exercise on steatohepatitis are unclear. This study aimed to clarify whether exercise training suppresses hepatic injury, inflammation, and fibrosis by suppressing macrophage infiltration. Male C57BL/6J (4-week old) mice were randomly divided into four groups: normal diet (ND) control (. n=. 7), ND exercise (. n=. 5), high-fat diet and high-fructose water (HFF) control (. n=. 11), and HFF exercise (. n=. 11) groups. Mice were fed the ND or HFF from 4 to 20. weeks of age. The exercise groups were trained on a motorized treadmill for 60. min/day, five times/week. The nonalcoholic fatty liver disease (NAFLD) activity score and plasma ALT activity, indicators of liver injury, were increased in HFF control mice but were attenuated in HFF exercise mice. Hepatic inflammation, indicated by hepatic tumor necrosis factor (TNF)-α levels and hepatic resident macrophage infiltration, was significantly lower in HFF exercise mice than in HFF control mice. Hepatic fibrosis markers (histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining and tissue inhibitor of matrix metalloproteinase-1 mRNA) were attenuated in HFF exercise mice compared with HFF control mice. These results suggest that exercise training reduces hepatic inflammation, injury, and fibrosis by suppressing macrophage infiltration.
AB - Nonalcoholic steatohepatitis, which is considered the hepatic event in metabolic syndrome, was recently associated with the innate immune system. Although regular exercise reduces hepatic injury markers like serum alanine aminotransferase (ALT) levels, the mechanisms regulating the effects of exercise on steatohepatitis are unclear. This study aimed to clarify whether exercise training suppresses hepatic injury, inflammation, and fibrosis by suppressing macrophage infiltration. Male C57BL/6J (4-week old) mice were randomly divided into four groups: normal diet (ND) control (. n=. 7), ND exercise (. n=. 5), high-fat diet and high-fructose water (HFF) control (. n=. 11), and HFF exercise (. n=. 11) groups. Mice were fed the ND or HFF from 4 to 20. weeks of age. The exercise groups were trained on a motorized treadmill for 60. min/day, five times/week. The nonalcoholic fatty liver disease (NAFLD) activity score and plasma ALT activity, indicators of liver injury, were increased in HFF control mice but were attenuated in HFF exercise mice. Hepatic inflammation, indicated by hepatic tumor necrosis factor (TNF)-α levels and hepatic resident macrophage infiltration, was significantly lower in HFF exercise mice than in HFF control mice. Hepatic fibrosis markers (histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining and tissue inhibitor of matrix metalloproteinase-1 mRNA) were attenuated in HFF exercise mice compared with HFF control mice. These results suggest that exercise training reduces hepatic inflammation, injury, and fibrosis by suppressing macrophage infiltration.
KW - Chemokine
KW - Exercise training
KW - Fatty liver
KW - Hepatic fibrosis
KW - Hepatic inflammation
KW - Macrophage
UR - http://www.scopus.com/inward/record.url?scp=84863873773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863873773&partnerID=8YFLogxK
U2 - 10.1016/j.bbi.2012.04.006
DO - 10.1016/j.bbi.2012.04.006
M3 - Article
VL - 26
SP - 931
EP - 941
JO - Brain, Behavior, and Immunity
JF - Brain, Behavior, and Immunity
SN - 0889-1591
IS - 6
ER -