Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses

Yo Kikuchi, Yumiko Ando, Nobuko Ichimura, Akihiro Noda

研究成果: Article

2 引用 (Scopus)

抜粋

Highly purified and commercially available preparations of reverse transcriptases from retroviruses contain a 3′ to 5′ exoribonuclease activity capable of hydrolyzing synthetic homopolyribonucleotides having a 3′ -OH end. The exoribonuclease activity of reverse transcriptase preparations from Rous associated virus-2 was further characterized. This exoribonuclease activity cleaves poly(C) and poly(U) exonucleolytically from the 3′-OH end to produce nucleoside 5′-phosphates. Poly(A), poly(G), circular polyribonucleotide, and double-stranded polyribonucleotide were not hydrolyzed by the activity. This is a novel type of exoribonuclease activity.

元の言語English
ページ(範囲)974-978
ページ数5
ジャーナルJournal of Biochemistry
105
発行部数6
出版物ステータスPublished - 1989 6
外部発表Yes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

フィンガープリント Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Kikuchi, Y., Ando, Y., Ichimura, N., & Noda, A. (1989). Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses. Journal of Biochemistry, 105(6), 974-978.