Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses

Yo Kikuchi*, Yumiko Ando, Nobuko Ichimura, Akihiro Noda

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Highly purified and commercially available preparations of reverse transcriptases from retroviruses contain a 3′ to 5′ exoribonuclease activity capable of hydrolyzing synthetic homopolyribonucleotides having a 3′ -OH end. The exoribonuclease activity of reverse transcriptase preparations from Rous associated virus-2 was further characterized. This exoribonuclease activity cleaves poly(C) and poly(U) exonucleolytically from the 3′-OH end to produce nucleoside 5′-phosphates. Poly(A), poly(G), circular polyribonucleotide, and double-stranded polyribonucleotide were not hydrolyzed by the activity. This is a novel type of exoribonuclease activity.

本文言語English
ページ(範囲)974-978
ページ数5
ジャーナルJournal of Biochemistry
105
6
出版ステータスPublished - 1989 6月
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生理学(医学)
  • 放射線学、核医学およびイメージング
  • 分子生物学
  • 生化学

フィンガープリント

「Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル