Expression characteristics and stimulatory functions of CD43 in human CD4+ memory T cells: Analysis using a monoclonal antibody to CD43 that has a novel lineage specificity

Seishi Kyoizumi*, Takaaki Ohara, Yoichiro Kusunoki, Tomonori Hayashi, Kazuaki Koyama, Naohiro Tsuyama

*この研究の対応する著者

研究成果: Article査読

11 被引用数 (Scopus)

抄録

We have used HSCA-2, an mAb that recognizes a sialic acid-dependent epitope on the low molecular mass (∼115-kDa) glycoform of CD43 that is expressed in resting T and NK cells, to examine the expression characteristics and stimulatory functions of CD43 in human CD4+ memory T cells. Having previously reported that the memory cells that respond to recall Ags in a CD4+CD45RO+ T cell population almost all belong to a subset whose surface CD43 expression levels are elevated, we now find that exposing these same memory T cells to HSCA-2 mAb markedly increases their proliferative responsiveness to recall Ags. We think it unlikely that this increase in responsiveness is a result of CD43-mediated monocyte activation, especially given that the HSCA-2 mAb differs from all previously used CD43 mAbs in having no obvious binding specificity for monocyte CD43. Predictably, treatment with HSCA-2 mAb did not lead to significant recall responses in CD4+CD45RO+ T cells, whose CD43 expression levels were similar to or lower than those of naive cells. Other experiments indicated that the HSCA-2 mAb was capable of enhancing the proliferative responsiveness of CD4+ memory T cells that had been exposed to polyclonal stimulation by monocyte-bound CD3 mAb and could also act in synergy with CD28 mAb to enhance the responsiveness of CD4+ T cells to CD3 stimulation. Taken together, these findings suggest that the CD43 molecules expressed on CD4 + memory T cells may be capable of enhancing the costimulatory signaling and hence providing accessory functions to TCR-mediated activation processes.

本文言語English
ページ(範囲)7246-7253
ページ数8
ジャーナルJournal of Immunology
172
12
DOI
出版ステータスPublished - 2004 6月 15
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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