Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria

Masahiro Ishiura, Shinsuke Kutsuna, Setsuyuki Aoki, Hideo Iwasaki, Carol R. Andersson, Akio Tanabe, Susan S. Golden, Carl H. Johnson, Takao Kondo

研究成果: Article査読

535 被引用数 (Scopus)

抄録

Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA and kaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. Continuous kaiC overexpression repressed the kaiBC promoter, whereas kaiA overexpression enhanced it. Temporal kaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.

本文言語English
ページ(範囲)1519-1523
ページ数5
ジャーナルScience
281
5382
DOI
出版ステータスPublished - 1998 9 4
外部発表はい

ASJC Scopus subject areas

  • 一般

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