Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster

Yoichi Ito, Atsushi Hirota, Mariko Nakamura, Masahiro Matsunaga, Kazuyoshi Tsutsui, Sakaé Kikuyama

研究成果: Article査読

抄録

As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.

本文言語English
ページ(範囲)348-350
ページ数3
ジャーナルAnnals of the New York Academy of Sciences
1040
DOI
出版ステータスPublished - 2005
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 科学史および科学哲学

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