Expression of Asn-d-Trp-Phe-NH2 in the brain of the terrestrial slug Limax valentianus

Ryota Matsuo*, Suguru Kobayashi, Fumihiro Morishita, Etsuro Ito

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The tripeptide Asn-d-Trp-Phe-NH2 (NdWFamide) is a d-amino acid-containing cardioexcitatory peptide initially isolated from Aplysia. Previously we detected NdWFamide immunoreactivity in the visceral giant cells, the largest neurons in the brain of the terrestrial slug Limax located at the dorsal surface of the visceral ganglia. In the present study, we further analyzed the morphological features of these neurons by an intracellular injection of Lucifer yellow, and found that these neurons extend neurites out of the brain through at least 5 nerve bundles. We then isolated a gene and a cDNA clone potentially encoding a NdWFamide precursor, and investigated expression at the levels of mRNA and protein in Limax. The NdWFamide gene consists of 5 exons spanning at least 17kb of the genome, and its open reading frame extends over 3 exons. The spatial expression pattern of NdWFamide mRNA was almost identical to that of the NdWFamide peptide, with some minor discrepancies in between. Although the most remarkable expression was evident in the visceral giant cells, we also found the expression of NdWFamide mRNA and peptide in the cerebral and pedal ganglia. These results suggest the involvement of NdWFamide in the regulation of a broad area of the slug's body.

本文言語English
ページ(範囲)89-93
ページ数5
ジャーナルComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
160
2-3
DOI
出版ステータスPublished - 2011 10月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 分子生物学

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