Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus

Takehiko Mima, Kazuyoshi Gotoh, Yumiko Yamamoto, Keiko Maeda, Taku Shirakawa, Shunsuke Matsui, Yumi Murata, Takaki Koide, Hiroshi Tokumitsu, Osamu Matsushita

    研究成果: Article

    2 引用 (Scopus)

    抄録

    Vibrio alginolyticus is an opportunistic pathogen in both humans and marine animals. Collagenase encoded by colA is considered to be one of the virulence factors. Expression of colA is regulated by multiple environmental factors, e.g., temperature, growth phase, and substrate. To elucidate the mechanism of regulation of colA expression, transposon mutagenesis was performed. VarS, a sensor histidine kinase of the two-component regulatory system, was demonstrated to regulate the expression of colA. VarA, a cognate response regulator of VarS, was also identified and shown to be involved in the regulation of colA expression. In vitro phosphorylation assays showed that phosphorylated VarS acted as a phosphoryl group donor to VarA. A site-directed mutagenesis study showed that the His300, Asp718 and His874 residues in VarS were essential for the phosphorylation of VarS, and the Asp54 residue in VarA was likely to receive the phosphoryl group from VarS. The results demonstrate that the VarS/VarA two-component regulatory system regulates the expression of collagenase in V. alginolyticus.

    元の言語English
    ページ(範囲)1-13
    ページ数13
    ジャーナルJournal of Membrane Biology
    DOI
    出版物ステータスAccepted/In press - 2017 10 9

    Fingerprint

    Vibrio alginolyticus
    Colicins
    Collagenases
    Phosphorylation
    Virulence Factors
    Site-Directed Mutagenesis
    Mutagenesis
    Temperature
    Growth

    ASJC Scopus subject areas

    • Biophysics
    • Physiology
    • Cell Biology

    これを引用

    Mima, T., Gotoh, K., Yamamoto, Y., Maeda, K., Shirakawa, T., Matsui, S., ... Matsushita, O. (受理済み/印刷中). Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. Journal of Membrane Biology, 1-13. https://doi.org/10.1007/s00232-017-9991-9

    Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. / Mima, Takehiko; Gotoh, Kazuyoshi; Yamamoto, Yumiko; Maeda, Keiko; Shirakawa, Taku; Matsui, Shunsuke; Murata, Yumi; Koide, Takaki; Tokumitsu, Hiroshi; Matsushita, Osamu.

    :: Journal of Membrane Biology, 09.10.2017, p. 1-13.

    研究成果: Article

    Mima, T, Gotoh, K, Yamamoto, Y, Maeda, K, Shirakawa, T, Matsui, S, Murata, Y, Koide, T, Tokumitsu, H & Matsushita, O 2017, 'Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus', Journal of Membrane Biology, pp. 1-13. https://doi.org/10.1007/s00232-017-9991-9
    Mima, Takehiko ; Gotoh, Kazuyoshi ; Yamamoto, Yumiko ; Maeda, Keiko ; Shirakawa, Taku ; Matsui, Shunsuke ; Murata, Yumi ; Koide, Takaki ; Tokumitsu, Hiroshi ; Matsushita, Osamu. / Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. :: Journal of Membrane Biology. 2017 ; pp. 1-13.
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    abstract = "Vibrio alginolyticus is an opportunistic pathogen in both humans and marine animals. Collagenase encoded by colA is considered to be one of the virulence factors. Expression of colA is regulated by multiple environmental factors, e.g., temperature, growth phase, and substrate. To elucidate the mechanism of regulation of colA expression, transposon mutagenesis was performed. VarS, a sensor histidine kinase of the two-component regulatory system, was demonstrated to regulate the expression of colA. VarA, a cognate response regulator of VarS, was also identified and shown to be involved in the regulation of colA expression. In vitro phosphorylation assays showed that phosphorylated VarS acted as a phosphoryl group donor to VarA. A site-directed mutagenesis study showed that the His300, Asp718 and His874 residues in VarS were essential for the phosphorylation of VarS, and the Asp54 residue in VarA was likely to receive the phosphoryl group from VarS. The results demonstrate that the VarS/VarA two-component regulatory system regulates the expression of collagenase in V. alginolyticus.",
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    AU - Maeda, Keiko

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    AU - Matsui, Shunsuke

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    AU - Koide, Takaki

    AU - Tokumitsu, Hiroshi

    AU - Matsushita, Osamu

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