Expression of mammalian mitochondrial F1-ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2

Toshiharu Suzuki, Naoya Iida, Junko Suzuki, Yasunori Watanabe, Toshiya Endo, Toru Hisabori, Masasuke Yoshida

研究成果: Article

抄録

F1-ATPase (F1) is a multisubunit water-soluble domain of FoF1-ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F1 assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are essential and sufficient for efficient production of recombinant bovine mitochondrial F1 in Escherichia coli cells. Intactness of the function and conformation of the E. coli-expressed bovine F1 was verified by rotation analysis and crystallization. This expression system opens a way for the previously unattempted mutation study of mammalian mitochondrial F1.

元の言語English
ページ(範囲)1267-1272
ページ数6
ジャーナルFEBS Open Bio
6
発行部数12
DOI
出版物ステータスPublished - 2016 12 1

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Proton-Translocating ATPases
Escherichia coli
Open systems
Crystallization
Yeast
Conformations
Adenosine Triphosphate
Yeasts
Mutation
Water
Enzymes
factor AF2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Expression of mammalian mitochondrial F1-ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2. / Suzuki, Toshiharu; Iida, Naoya; Suzuki, Junko; Watanabe, Yasunori; Endo, Toshiya; Hisabori, Toru; Yoshida, Masasuke.

:: FEBS Open Bio, 巻 6, 番号 12, 01.12.2016, p. 1267-1272.

研究成果: Article

Suzuki, Toshiharu ; Iida, Naoya ; Suzuki, Junko ; Watanabe, Yasunori ; Endo, Toshiya ; Hisabori, Toru ; Yoshida, Masasuke. / Expression of mammalian mitochondrial F1-ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2. :: FEBS Open Bio. 2016 ; 巻 6, 番号 12. pp. 1267-1272.
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