Expression of mammalian mitochondrial F1-ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2

Toshiharu Suzuki*, Naoya Iida, Junko Suzuki, Yasunori Watanabe, Toshiya Endo, Toru Hisabori, Masasuke Yoshida

*この研究の対応する著者

研究成果: Article査読

5 被引用数 (Scopus)

抄録

F1-ATPase (F1) is a multisubunit water-soluble domain of FoF1-ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F1 assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are essential and sufficient for efficient production of recombinant bovine mitochondrial F1 in Escherichia coli cells. Intactness of the function and conformation of the E. coli-expressed bovine F1 was verified by rotation analysis and crystallization. This expression system opens a way for the previously unattempted mutation study of mammalian mitochondrial F1.

本文言語English
ページ(範囲)1267-1272
ページ数6
ジャーナルFEBS Open Bio
6
12
DOI
出版ステータスPublished - 2016 12 1

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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