Expression of rfamide-related peptide in the dorsomedial nucleus of hypothalamus during the estrous cycle of rats

Mohammad Reza Jafarzadeh Shirazi, Farid Pazhoohi, Mohammad Javad Zamiri, Mohammad Saied Salehi, Mohammad Reza Namavar, Amin Tamadon, Nader Tanideh, Afsoon Zarei, Kazuyoshi Tsutsui

    研究成果: Article査読

    10 被引用数 (Scopus)

    抄録

    Introduction: RFamide-related peptide (RFRP) is believed to act as an inhibitor of gonadotropin releasing hormone (GnRH) secretion. The aim of the present study was to compare the expression pattern of RFRP neurons in the dorsomedial nucleus of hypothalamus (DMH) at different phases of the rat estrous cycle. Methods: The phases of the estrous cycle were determined in 16 adult female Sprague-Dawley rats using vaginal smears. The rats were divided into five groups: proestrus phase (n=4), early estrus phase (n=3), estrus phase (n=3), metestrus phase (n=3) and diestrus phase (n=3). After transcardial perfusion, their brains were removed and fixed. Diencephalon of each rat brain was sectioned, and DMH-containing sections were stained using an immunohistochemical method. The number of RFRP positive neurons in DMH was counted microscopically. Results: Almost all of the neurons expressing RFRP in the DMH were bipolar. Mean and standard error of the number of RFRP neurons during diestrus (45.8±12.6) and estrus phases (44.7±3.6) were greater (P<0.05) than early estrus (18.8±0.8) and proestrus phases (16.2±2.0). Conclusion: Results confirm a regulatory role for RFRP in DMH in the control of rat estrous cycle.

    本文言語English
    ページ(範囲)72-79
    ページ数8
    ジャーナルPhysiology and Pharmacology
    17
    1
    出版ステータスPublished - 2013

    ASJC Scopus subject areas

    • Pharmacology
    • Physiology

    フィンガープリント 「Expression of rfamide-related peptide in the dorsomedial nucleus of hypothalamus during the estrous cycle of rats」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル