Expression of the green fluorescent protein derivative S65T in Xenopus laevis oocytes

Julie M. Matheson, Atsushi Miyawaki, Akira Muto*, Takafumi Inoue, Katsuhiko Mikoshiba

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The coding region of the green fluorescent protein mutant S65T has been fused to the eukaryotic expression vector pMT3 and injected into the nucleus of Xenopus laevis oocytes. The fluorescent gene product is observed within 24-48 h and can be used as an internal control to monitor gene expression without the need for added substrates. Co-injection of pMT3-S65T and a pMT3 construct containing the coding region of the mouse cerebellar type 1 inositol 1,4,5-trisphosphate receptor (IP3R) showed that the fluorescence level of S65T paralleled expression of IP3R. This correlation makes it possible to visually evaluate relative levels of IP3R expression.

本文言語English
ページ(範囲)221-225
ページ数5
ジャーナルBiomedical Research
17
3
DOI
出版ステータスPublished - 1996 6
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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