Expression of the melatonin receptor Mel1c in neural tissues of the reef fish Siganus guttatus

Yong Ju Park, Ji Gweon Park, Hyung Bok Jeong, Yuki Takeuchi, Se Jae Kim, Young Don Lee, Akihiro Takemura*

*この研究の対応する著者

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The golden rabbitfish, Siganus guttatus, is a reef fish exhibiting a restricted lunar-related rhythm in behavior and reproduction. Here, to understand the circadian rhythm of this lunar-synchronized spawner, a melatonin receptor subtype-Mel1c-was cloned. The full-length Mel1c melatonin receptor cDNA comprised 1747 bp with a single open reading frame (1062 bp) that encodes a 353-amino acid protein, which included 7 presumed transmembrane domains. Real-time PCR revealed high Mel1c mRNA expression in the retina and brain but not in the peripheral tissues. When the fish were reared under light/dark (LD 12:12) conditions, Mel1c mRNA in the retina and brain was expressed with daily variations and increased during nighttime. Similar variations were noted under constant conditions, suggesting that Mel1c mRNA expression is regulated by the circadian clock system. Daily variations of Mel1c mRNA expression with a peak at zeitgeber time (ZT) 12 were observed in the cultured pineal gland under LD 12:12. Exposure of the cultured pineal gland to light at ZT17 resulted in a decrease in Mel1c mRNA expression. When light was obstructed at ZT5, the opposite effect was obtained. These results suggest that light exerts certain effects on Mel1c mRNA expression directly or indirectly through melatonin actions.

本文言語English
ページ(範囲)103-111
ページ数9
ジャーナルComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
147
1
DOI
出版ステータスPublished - 2007 5
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 生理学

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