Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet

Daichi Someya, Satoshi Arai, Toshinori Fujie, Shinji Takeoka

研究成果: Article

1 引用 (Scopus)

抄録

We have developed a sheet-like pH imaging sensor based on a flexible and physically adhesive polymer thin film (referred to as a “pH sensing nanosheet”). The pH sensing nanosheet was composed of two films: one is a pH-sensitive layer-by-layer (LbL) film constructed from fluorescein-conjugated poly(acrylic acid) and poly(allylamine hydrochloride) and the other is a pH-insensitive film made from Nile red-embedded poly(d,l-lactic acid). The pH sensing nanosheet enabled the ratiometric imaging of pH changes in a leaf (500 × 500 μm2), namely the apoplastic ion milieu responding to an external NaCl stress. It was successfully mapped out that the alkalization of the leaf apoplast spread from the leaf base to the tip at 20 min after the stimulation and the pH value increased up to approximately pH 6.3 from less than pH 4.5 within 60 min when a 100 mM NaCl aqueous solution was added. The pH sensing nanosheet should be useful for energy metabolic mapping in tissue biology.

元の言語English
ページ(範囲)35651-35657
ページ数7
ジャーナルRSC Advances
8
発行部数62
DOI
出版物ステータスPublished - 2018 1 1

Fingerprint

Nanosheets
Polyelectrolytes
Imaging techniques
Lactic acid
Fluorescein
Polymer films
Acrylics
Adhesives
Ions
Tissue
Thin films
Acids
Sensors

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

これを引用

Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet. / Someya, Daichi; Arai, Satoshi; Fujie, Toshinori; Takeoka, Shinji.

:: RSC Advances, 巻 8, 番号 62, 01.01.2018, p. 35651-35657.

研究成果: Article

Someya, Daichi ; Arai, Satoshi ; Fujie, Toshinori ; Takeoka, Shinji. / Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet. :: RSC Advances. 2018 ; 巻 8, 番号 62. pp. 35651-35657.
@article{4ec3cb82655240fc9dc718f0357f2728,
title = "Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet",
abstract = "We have developed a sheet-like pH imaging sensor based on a flexible and physically adhesive polymer thin film (referred to as a “pH sensing nanosheet”). The pH sensing nanosheet was composed of two films: one is a pH-sensitive layer-by-layer (LbL) film constructed from fluorescein-conjugated poly(acrylic acid) and poly(allylamine hydrochloride) and the other is a pH-insensitive film made from Nile red-embedded poly(d,l-lactic acid). The pH sensing nanosheet enabled the ratiometric imaging of pH changes in a leaf (500 × 500 μm2), namely the apoplastic ion milieu responding to an external NaCl stress. It was successfully mapped out that the alkalization of the leaf apoplast spread from the leaf base to the tip at 20 min after the stimulation and the pH value increased up to approximately pH 6.3 from less than pH 4.5 within 60 min when a 100 mM NaCl aqueous solution was added. The pH sensing nanosheet should be useful for energy metabolic mapping in tissue biology.",
author = "Daichi Someya and Satoshi Arai and Toshinori Fujie and Shinji Takeoka",
year = "2018",
month = "1",
day = "1",
doi = "10.1039/C8RA06308G",
language = "English",
volume = "8",
pages = "35651--35657",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "62",

}

TY - JOUR

T1 - Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet

AU - Someya, Daichi

AU - Arai, Satoshi

AU - Fujie, Toshinori

AU - Takeoka, Shinji

PY - 2018/1/1

Y1 - 2018/1/1

N2 - We have developed a sheet-like pH imaging sensor based on a flexible and physically adhesive polymer thin film (referred to as a “pH sensing nanosheet”). The pH sensing nanosheet was composed of two films: one is a pH-sensitive layer-by-layer (LbL) film constructed from fluorescein-conjugated poly(acrylic acid) and poly(allylamine hydrochloride) and the other is a pH-insensitive film made from Nile red-embedded poly(d,l-lactic acid). The pH sensing nanosheet enabled the ratiometric imaging of pH changes in a leaf (500 × 500 μm2), namely the apoplastic ion milieu responding to an external NaCl stress. It was successfully mapped out that the alkalization of the leaf apoplast spread from the leaf base to the tip at 20 min after the stimulation and the pH value increased up to approximately pH 6.3 from less than pH 4.5 within 60 min when a 100 mM NaCl aqueous solution was added. The pH sensing nanosheet should be useful for energy metabolic mapping in tissue biology.

AB - We have developed a sheet-like pH imaging sensor based on a flexible and physically adhesive polymer thin film (referred to as a “pH sensing nanosheet”). The pH sensing nanosheet was composed of two films: one is a pH-sensitive layer-by-layer (LbL) film constructed from fluorescein-conjugated poly(acrylic acid) and poly(allylamine hydrochloride) and the other is a pH-insensitive film made from Nile red-embedded poly(d,l-lactic acid). The pH sensing nanosheet enabled the ratiometric imaging of pH changes in a leaf (500 × 500 μm2), namely the apoplastic ion milieu responding to an external NaCl stress. It was successfully mapped out that the alkalization of the leaf apoplast spread from the leaf base to the tip at 20 min after the stimulation and the pH value increased up to approximately pH 6.3 from less than pH 4.5 within 60 min when a 100 mM NaCl aqueous solution was added. The pH sensing nanosheet should be useful for energy metabolic mapping in tissue biology.

UR - http://www.scopus.com/inward/record.url?scp=85055434476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055434476&partnerID=8YFLogxK

U2 - 10.1039/C8RA06308G

DO - 10.1039/C8RA06308G

M3 - Article

VL - 8

SP - 35651

EP - 35657

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 62

ER -