Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase

T. Tanii*, T. Goto, T. Iida, M. Koh-Masahara, I. Ohdomari

*この研究の対応する著者

研究成果: Letter査読

22 被引用数 (Scopus)

抄録

We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.

本文言語English
ページ(範囲)L1135-L1137
ジャーナルJapanese Journal of Applied Physics, Part 2: Letters
40
10 B
DOI
出版ステータスPublished - 2001 10月 15

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(その他)
  • 物理学および天文学(全般)

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