Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase

Takashi Tanii, T. Goto, T. Iida, M. Koh-Masahara, I. Ohdomari

    研究成果: Article

    20 引用 (Scopus)

    抜粋

    We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.

    元の言語English
    ジャーナルJapanese Journal of Applied Physics, Part 2: Letters
    40
    発行部数10 B
    出版物ステータスPublished - 2001 10 15

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    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

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