Fabrication of high-aspect-ratio amorphous perfluorinated polymer structure for total internal reflection fluorescence microscopy

Asahi Nakahara, Yoshitaka Shirasaki, Kentaro Kawai, Osamu Ohara, Jun Mizuno, Shuichi Shoji

研究成果: Article

5 引用 (Scopus)

抄録

A method to fabricate microstructures that are ideal for optical applications was developed. This method enabled structures with a high aspect ratio to be fabricated from an amorphous perfluorinated polymer, CYTOP, which has an identical refractive index to that of water. Using the method, a microwell array device was developed for the purpose of high-throughput protein quantification. Evaluation of the optical properties of the array using total internal reflection fluorescence (TIRF) microscopy demonstrated that the device did not interfere with TIRF imaging at the bottom of each microwell. This method can expand the possible applications of biophotonic devices as well as TIRF imaging.

元の言語English
ページ(範囲)1817-1820
ページ数4
ジャーナルMicroelectronic Engineering
88
発行部数8
DOI
出版物ステータスPublished - 2011 8

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Fluorescence microscopy
high aspect ratio
Aspect ratio
Polymers
microscopy
Fabrication
fluorescence
fabrication
polymers
Fluorescence
Imaging techniques
Refractive index
Optical properties
Throughput
refractivity
proteins
Proteins
optical properties
microstructure
Microstructure

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics

これを引用

Fabrication of high-aspect-ratio amorphous perfluorinated polymer structure for total internal reflection fluorescence microscopy. / Nakahara, Asahi; Shirasaki, Yoshitaka; Kawai, Kentaro; Ohara, Osamu; Mizuno, Jun; Shoji, Shuichi.

:: Microelectronic Engineering, 巻 88, 番号 8, 08.2011, p. 1817-1820.

研究成果: Article

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AU - Mizuno, Jun

AU - Shoji, Shuichi

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