Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

Yumi Yamahara*, Naotsugu Nagasawa, Mitsumasa Taguchi, Akihiro Oshima, Masakazu Washio

*この研究の対応する著者

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (–CF2–CF(OO・)–) on tetrafluoroethylene unit in ETFE.

本文言語English
ページ(範囲)88-93
ページ数6
ジャーナルRadiation Physics and Chemistry
142
DOI
出版ステータスPublished - 2018 1月

ASJC Scopus subject areas

  • 放射線

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