Fast and sensitive bacteria detection by boronic acid modified fluorescent dendrimer

Ayame Mikagi, Riho Tsurufusa, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita*

*この研究の対応する著者

研究成果: Article査読

抄録

This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 104 colony-forming units (CFU)·mL−1 . Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria’s surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria.

本文言語English
論文番号3115
ジャーナルSensors
21
9
DOI
出版ステータスPublished - 2021 5 1

ASJC Scopus subject areas

  • 分析化学
  • 情報システム
  • 原子分子物理学および光学
  • 生化学
  • 器械工学
  • 電子工学および電気工学

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