Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Muneki Isokawa, Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

研究成果: Conference contribution

抄録

A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

元の言語English
ホスト出版物のタイトル18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版者Chemical and Biological Microsystems Society
ページ941-943
ページ数3
ISBN(印刷物)9780979806476
出版物ステータスPublished - 2014
イベント18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
継続期間: 2014 10 262014 10 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
United States
San Antonio
期間14/10/2614/10/30

Fingerprint

Plasmas
Liquid chromatography
Biomarkers
Screening

ASJC Scopus subject areas

  • Control and Systems Engineering

これを引用

Isokawa, M., Song, Y., Takatsuki, K., Sekiguchi, T., Mizuno, J., Funatsu, T., ... Tsunoda, M. (2014). Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. : 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 941-943). Chemical and Biological Microsystems Society.

Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. / Isokawa, Muneki; Song, Yanting; Takatsuki, Katsuya; Sekiguchi, Tetsushi; Mizuno, Jun; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 941-943.

研究成果: Conference contribution

Isokawa, M, Song, Y, Takatsuki, K, Sekiguchi, T, Mizuno, J, Funatsu, T, Shoji, S & Tsunoda, M 2014, Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. : 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 941-943, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.
Isokawa M, Song Y, Takatsuki K, Sekiguchi T, Mizuno J, Funatsu T その他. Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. : 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 941-943
Isokawa, Muneki ; Song, Yanting ; Takatsuki, Katsuya ; Sekiguchi, Tetsushi ; Mizuno, Jun ; Funatsu, Takashi ; Shoji, Shuichi ; Tsunoda, Makoto. / Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 941-943
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AU - Funatsu, Takashi

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