Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Muneki Isokawa, Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda*

*この研究の対応する著者

研究成果: Conference contribution

抄録

A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

本文言語English
ホスト出版物のタイトル18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版社Chemical and Biological Microsystems Society
ページ941-943
ページ数3
ISBN(印刷版)9780979806476
出版ステータスPublished - 2014
イベント18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
継続期間: 2014 10 262014 10 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
国/地域United States
CitySan Antonio
Period14/10/2614/10/30

ASJC Scopus subject areas

  • 制御およびシステム工学

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