Fatty acid synthesis of an eicosapentaenoic acid-producing bacterium: De novo synthesis, chain elongation, and desaturation systems

Kazuo Watanabe*, Kazunaga Yazawa, Kiyosi Kondo, Akihiko Kawaguchi

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

The fatty acid synthesis systems of a Shewanella sp., strain SCRC-2738, that produces a large amount of eicosapentaenoic acid were investigated. Two kinds of fatty acid synthesis system, de novo synthesis and chain elongation ones, were detected in the cytosol. The de novo synthesis system required an acyl carrier protein, and produced palmitoyl- and palmitoleoyl-acyl carrier proteins as final products. The chain elongation system also required an acyl carrier protein, and produced an acyl-acyl carrier protein as a product, using palmitoyl-, palmitoleoyl-, stearoyl-, and oleoyl-CoAs as primers but not eicosanoyl- or eicosenoyl-CoA. There were an anaerobic pathway and an aerobic desaturation one for the production of unsaturated fatty acids. Eicosapentaenoic acid seemed to be produced through the aerobic desaturation pathway and not through the anaerobic one, since the latter pathway produced n-7 type monoenoic fatty acids, which are different from eicosapentaenoic acid in the position of the double bond. The desaturase utilized an acyl-acyl carrier protein as a substrate, and this activity increased in the presence of ferredoxin and ferredoxin NADP+ reductase. Thus, Shewanella sp., strain SCRC-2738, has novel characteristics as to both fatty acid chain elongation and desaturation systems.

本文言語English
ページ(範囲)467-473
ページ数7
ジャーナルJournal of Biochemistry
122
2
出版ステータスPublished - 1997 8月
外部発表はい

ASJC Scopus subject areas

  • 生化学

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