Fiber-optic sensor with a sandwich binding technique for fluoroimmunoassay

Noriyuki Nakamura, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

We report the development of a fiber-optic sensor based on the principle of the sandwich binding technique for the fluoro-immunoassay. Anti-mouse immunoglobulin G (IgG) antibody was immobilized on the membrane mounted to the edge of the fiber. The sensor was exposed to the solution containing mouse IgG and then allophycocyanin conjugated anti-mouse IgG antibody was added. The helium-neon (He-Ne) laser provides excitation of the sandwich binding antigen-antibody complex. This results in fluorescence emission at the membrane. Increase in the fluorescence intensity from sensing tip was proportional to the amount of mouse IgG in the sample. A linear relationship was obtained betweenthe fluorescence intensity increase and the mouse IgG concentration in the range 0.3 - 9.0 pg/ml.

本文言語English
ページ(範囲)1075-1084
ページ数10
ジャーナルAnalytical Letters
24
7
DOI
出版ステータスPublished - 1991 7 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 分光学
  • 電気化学
  • 生化学、医学
  • 臨床生化学
  • 生化学

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