Fibronectin-calcium phosphate composite layer on hydroxyapatite to enhance adhesion, cell spread and osteogenic differentiation of human mesenchymal stem cells in vitro

Yu Sogo*, Atsuo Ito, Tomonori Matsuno, Ayako Oyane, Gaku Tamazawa, Tazuko Satoh, Atsushi Yamazaki, Eiji Uchimura, Tadao Ohno

*この研究の対応する著者

研究成果: Article査読

40 被引用数 (Scopus)

抄録

Fibronectin (Fn) and type I collagen (Col) were immobilized on a surface of a hydroxyapatite (HAP) ceramic by coprecipitation with calcium phosphate in a supersaturated calcium phosphate solution prepared by mixing clinically approved infusion fluids. These proteins and the calcium phosphate precipitate formed a composite surface layer. As a result, the proteins were immobilized firmly as not to be released completely for 3 d in a physiological salt solution. When human mesenchymal stem cells (hMSCs) were cultured on a HAP ceramic in a differentiation medium supplemented with dexamethasone, β-glycerophosphate and ascorbic acid, hMSCs spread well within 1 h. The alkaline phosphatase (ALP) activity of hMSCs cultured on the Fn-calcium phosphate composite layer significantly increased compared with that of hMSCs cultured on the untreated HAP ceramic. On the other hand, Col did not increase the ALP activity of hMSCs and no synergy between Fn and Col was observed. Therefore, the Fn-calcium phosphate composite layer formed on the HAP is useful for the enhancement of the spreading and osteogenic differentiation of hMSCs in vitro.

本文言語English
論文番号009
ページ(範囲)116-123
ページ数8
ジャーナルBiomedical Materials
2
2
DOI
出版ステータスPublished - 2007 6月 1

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生体材料
  • 生体医工学

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