TY - JOUR
T1 - Flow immunoassay for detection of human chorionic gonadotrophin using a cation exchange resin packed capillary column
AU - Nakamura, Noriyuki
AU - Lim, Tae Kyu
AU - Jeong, Jae Mun
AU - Matsunaga, Tadashi
PY - 2001/7/17
Y1 - 2001/7/17
N2 - A human chorionic gonadotrophin (HCG) detection was carried out by the flow immunoassay system with a cation exchange resin packed capillary column (762 μm i.d. × 10 cm length), that can be considered a miniaturised version of the previously reported system. The HCG and fluorescein isothiocyanate (FITC) conjugated immunoglobulin G (IgG) antibody complex was separated from free FITC conjugated IgG on the basis of differences in isoelectric points. The fluorescence intensity correlated linearly with concentrations of HCG in the range of 25-1500 mIU/ml. This simple technique permits the assay of HCG within 1.3 min. It was possible to reuse of free FITC conjugated IgG. This simple and rapid immunoassay method can be used for the continuous detection of HCG.
AB - A human chorionic gonadotrophin (HCG) detection was carried out by the flow immunoassay system with a cation exchange resin packed capillary column (762 μm i.d. × 10 cm length), that can be considered a miniaturised version of the previously reported system. The HCG and fluorescein isothiocyanate (FITC) conjugated immunoglobulin G (IgG) antibody complex was separated from free FITC conjugated IgG on the basis of differences in isoelectric points. The fluorescence intensity correlated linearly with concentrations of HCG in the range of 25-1500 mIU/ml. This simple technique permits the assay of HCG within 1.3 min. It was possible to reuse of free FITC conjugated IgG. This simple and rapid immunoassay method can be used for the continuous detection of HCG.
KW - Capillary column
KW - Continuous detection
KW - Flow immunoassay
KW - Fluorescein isothiocyanate conjugated immunoglobulin G
KW - Human chorionic gonadotrophin
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U2 - 10.1016/S0003-2670(01)01012-1
DO - 10.1016/S0003-2670(01)01012-1
M3 - Article
AN - SCOPUS:0035902634
VL - 439
SP - 125
EP - 130
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -