Flow immunoassay for detection of human chorionic gonadotrophin using a cation exchange resin packed capillary column

Noriyuki Nakamura, Tae Kyu Lim, Jae Mun Jeong, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

26 被引用数 (Scopus)

抄録

A human chorionic gonadotrophin (HCG) detection was carried out by the flow immunoassay system with a cation exchange resin packed capillary column (762 μm i.d. × 10 cm length), that can be considered a miniaturised version of the previously reported system. The HCG and fluorescein isothiocyanate (FITC) conjugated immunoglobulin G (IgG) antibody complex was separated from free FITC conjugated IgG on the basis of differences in isoelectric points. The fluorescence intensity correlated linearly with concentrations of HCG in the range of 25-1500 mIU/ml. This simple technique permits the assay of HCG within 1.3 min. It was possible to reuse of free FITC conjugated IgG. This simple and rapid immunoassay method can be used for the continuous detection of HCG.

本文言語English
ページ(範囲)125-130
ページ数6
ジャーナルAnalytica Chimica Acta
439
1
DOI
出版ステータスPublished - 2001 7月 17
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分析化学
  • 分光学
  • 環境化学

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