Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim*, Noriyuki Nakamura, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

本文言語English
ページ(範囲)445-449
ページ数5
ジャーナルBiotechnology Techniques
12
6
DOI
出版ステータスPublished - 1998
外部発表はい

ASJC Scopus subject areas

  • 応用微生物学とバイオテクノロジー
  • 生化学

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