抄録
A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.
本文言語 | English |
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ページ(範囲) | 445-449 |
ページ数 | 5 |
ジャーナル | Biotechnology Techniques |
巻 | 12 |
号 | 6 |
DOI | |
出版ステータス | Published - 1998 |
外部発表 | はい |
ASJC Scopus subject areas
- 応用微生物学とバイオテクノロジー
- 生化学