抄録
An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 29-34 |
| ページ数 | 6 |
| ジャーナル | Analytica Chimica Acta |
| 巻 | 354 |
| 号 | 1-3 |
| DOI | |
| 出版ステータス | Published - 1997 11月 10 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 生化学
- 分析化学
- 分光学
- 環境化学