Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga

研究成果: Article

12 引用 (Scopus)

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An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.

元の言語English
ページ(範囲)29-34
ページ数6
ジャーナルAnalytica Chimica Acta
354
発行部数1-3
DOI
出版物ステータスPublished - 1997 11 10
外部発表Yes

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ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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