Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

Kazuhiro Furukawa, Hiroshi Abe, Naoko Abe, Mitsuru Harada, Satoshi Tsuneda, Yoshihiro Ito

研究成果: Article

9 引用 (Scopus)

抜粋

We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

元の言語English
ページ(範囲)4562-4565
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
18
発行部数16
DOI
出版物ステータスPublished - 2008 8 15

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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