TY - JOUR
T1 - Fluorescence imaging of Escherichia coli on a rotating optical disk
AU - Shima, Takayuki
AU - Shiramizu, Hideyuki
AU - Fujimaki, Makoto
AU - Kuroda, Chiaki
AU - Ohki, Yoshimichi
N1 - Funding Information:
Acknowledgments The authors would like to thank Dr. X. Wang, formerly belonging to AIST, for his help on development of the optical setup. This work was partly supported by JSPS KAKENHI Grant Number 25246033.
Publisher Copyright:
© 2018 The Japan Society of Applied Physics.
PY - 2018/8
Y1 - 2018/8
N2 - We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.
AB - We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.
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U2 - 10.7567/JJAP.57.088003
DO - 10.7567/JJAP.57.088003
M3 - Article
AN - SCOPUS:85050966668
SN - 0021-4922
VL - 57
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
IS - 8
M1 - 088003
ER -