Fluorogenic probe triggered by reduction for nucleic acids sensing.

Kazuhiro Furukawa, Hiroshi Abe, Jin Wang, Kazuma Oki, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito

研究成果: Article査読


A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.

ジャーナルNucleic acids symposium series (2004)
出版ステータスPublished - 2008 12 1

ASJC Scopus subject areas

  • Medicine(all)

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