Fluorometric observation of viable and dead adhering diatoms using TO- PRO-1 iodide and its application to the estimation of electrochemical treatment

M. Okochi, T. Taguchi, M. Tsuboi, N. Nakamura, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

7 被引用数 (Scopus)

抄録

A rapid method for the direct measurement of viable and dead adhering diatoms was developed using a fluorescent dye, TO-PRO-1 iodide. By staining the marine diatom, Nitzchia closterium, with TO-PRO-1 iodide, viable and dead cells were identified as red and yellow cells respectively, under an epifluorescence microscope employing blue excitation. Only dead cells were stained with TO-PRO-1 iodide. Viable cells were observed as red because of autofluorescence arising from intracellular chlorophyll, whereas dead cells were observed as yellow because of the fluorescence of TO-PRO-1 iodide. The percentage of TO-PRO-1-iodide-stained was correlated with the percentage of dead cells in N. closterium cells exposed to heat (60 °C, 15 min). Other microalgae containing intracellular chlorophyll could be also distinguished as viable or dead cells by this fluorometric staining method. This method was applied for the assessment of N. closterium cells killed by the electrochemical treatment and used to monitor biofouling populations and their viability directly on the electrode surface. When 1.0 V was applied against a saturated calomel electrode, 99% of the cells attached to graphite electrode were killed in 1 h.

本文言語English
ページ(範囲)364-369
ページ数6
ジャーナルApplied Microbiology and Biotechnology
51
3
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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