Foreign gene expression in marine cyanobacteria under pseudo-continuous culture

Koji Sode*, Masahiro Tatara, Naoaki Hatano, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230. CAT has been successfully expressed in marine cyanobacteria under kanamycin resistance gene promoter. Pseudo-continuous culture of these recombinant marine cyanobacteria has been done by varying the dilution rate. At a dilution rate between 0.01 to 0.02 h-1, highest productivity was achieved. Under these conditions, long-term pseudo-continuous culture of recombinant marine cyanobacteria was achieved for more than 600 h with CAT productivity 18-fold greater than that observed in batch culture.

本文言語English
ページ(範囲)243-248
ページ数6
ジャーナルJournal of Biotechnology
33
3
DOI
出版ステータスPublished - 1994 4 15
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー

フィンガープリント

「Foreign gene expression in marine cyanobacteria under pseudo-continuous culture」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル