Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer

Brandon Yoza, Atsushi Arakaki, Kohei Maruyama, Haruko Takeyama, Tadashi Matsunaga

研究成果: Article査読

77 被引用数 (Scopus)

抄録

Bacterial and artificial magnetic particles were modified using a polyamidoamine (PAMAM) dendrimer and outer shell amines determined. Bacterial magnetic particles were the most consistently modified. Transmission electron microscopic (TEM) analysis showed that the artificial magnetic particles were structurally damaged by the modification process including sonication. Furthermore, laser particle analysis of the magnetite also revealed damage. Small quantities of dendrimer-modified bacterial magnetic particles were used to extract DNA from blood. The efficiency of DNA recovery was consistently about 30 ng of DNA using 2-10 μg of dendrimer-modified bacterial magnetite. This technique was fully automated using newly developed liquid handling robots and bacterial magnetic particles.

本文言語English
ページ(範囲)21-26
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
95
1
DOI
出版ステータスPublished - 2003
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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