Function of fibrinogen γ-chain dodecapeptide-conjugated latex beads under flow

Shinji Takeoka, Yosuke Okamura, Yuji Teramura, Naohide Watanabe, Hidenori Suzuki, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

    研究成果: Article

    28 引用 (Scopus)

    抄録

    In order to perform a fundamental study of platelet substitutes, novel particles that bound to activated platelets were prepared using two oligopeptides conjugated to latex beads. The oligopeptides were CHHLGGAKQAGDV (H12), which is a fibrinogen γ-chain carboxy-terminal sequence (γ 400-411), and CGGRGDF (RGD), which contains a fibrinogen α-chain sequence (α 95-98 RGDF). Both peptides contained an additional amino-terminal cysteine to enable conjugation. Human serum albumin was adsorbed onto the surface of latex beads (average diameter 1μm) and pyridyldisulfide groups were chemically introduced into the adsorbed protein. H12 or RGD peptides were then chemically linked to the modified surface protein via disulfide linkages. H12- or RGD-conjugated latex beads prepared in this way enhanced the in vitro thrombus formation of activated platelets on collagen-immobilized plates under flowing thrombocytopenic-imitation blood. Based on the result of flow cytometric analyses of agglutination, PAC-1 binding, antiP-selectin antibody binding, and annexin V binding, the H12-conjugated latex beads showed minimal interaction with non-activated platelets. These results indicate the excellent potential of H12-conjugated particles as a candidate for a platelet substitute.

    元の言語English
    ページ(範囲)773-779
    ページ数7
    ジャーナルBiochemical and Biophysical Research Communications
    312
    発行部数3
    DOI
    出版物ステータスPublished - 2003 12 19

    Fingerprint

    Latex
    Platelets
    Microspheres
    Fibrinogen
    Blood Platelets
    Oligopeptides
    Selectins
    Annexin A5
    Agglutination
    Serum Albumin
    Disulfides
    Cysteine
    Membrane Proteins
    Thrombosis
    Blood
    Collagen
    Peptides
    Antibodies
    Proteins

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    これを引用

    Function of fibrinogen γ-chain dodecapeptide-conjugated latex beads under flow. / Takeoka, Shinji; Okamura, Yosuke; Teramura, Yuji; Watanabe, Naohide; Suzuki, Hidenori; Tsuchida, Eishun; Handa, Makoto; Ikeda, Yasuo.

    :: Biochemical and Biophysical Research Communications, 巻 312, 番号 3, 19.12.2003, p. 773-779.

    研究成果: Article

    Takeoka, S, Okamura, Y, Teramura, Y, Watanabe, N, Suzuki, H, Tsuchida, E, Handa, M & Ikeda, Y 2003, 'Function of fibrinogen γ-chain dodecapeptide-conjugated latex beads under flow', Biochemical and Biophysical Research Communications, 巻. 312, 番号 3, pp. 773-779. https://doi.org/10.1016/j.bbrc.2003.10.184
    Takeoka, Shinji ; Okamura, Yosuke ; Teramura, Yuji ; Watanabe, Naohide ; Suzuki, Hidenori ; Tsuchida, Eishun ; Handa, Makoto ; Ikeda, Yasuo. / Function of fibrinogen γ-chain dodecapeptide-conjugated latex beads under flow. :: Biochemical and Biophysical Research Communications. 2003 ; 巻 312, 番号 3. pp. 773-779.
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