Functional analysis of lysosomes during mouse preimplantation embryo development

Satoshi Tsukamoto, Taichi Hara, Atsushi Yamamoto, Yuki Ohta, Ayako Wada, Yuka Ishida, Seiji Kito, Tetsu Nishikawa, Naojiro Minami, Ken Sato, Toshiaki Kokubo

研究成果: Article査読

27 被引用数 (Scopus)


Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers decreased slightly in blastocysts. Consistently, the protein expression level of mature cathepsins B and D was high from the one-cell to morula stages but low in the blastocyst stage. One-cell embryos injected with siRNAs targeted to both lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) were developmentally arrested at the two-cell stage. Pharmacological inhibition of lysosomes also caused developmental retardation, resulting in accumulation of lipofuscin. Our fndings highlight the functional changes in lysosomes in mouse preimplantation embryos.

ジャーナルJournal of Reproduction and Development
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • 動物科学および動物学


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