Functional domains for assembly of histones H3 and H4 into the chromatin of Xenopus embryos

Lita Freeman, Hitoshi Kurumizaka, Alan P. Wolffe*

*この研究の対応する著者

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Histones H3 and H4 have a well defined structural role in the nucleosome and an established role in the regulation of transcription. We have made use of a microinjection strategy using Xenopus embryos to define the minimal structural components of H3 and H4 necessary for nucleosome assembly into metazoan chromosomes in vivo. We find that both the N-terminal tail of H4, including all sites of acetylation, and the C-terminal α-helix of the H4 histone fold domain are dispensable for chromatin assembly. The N-terminal tail and an N-terminal α-helix of H3 are also dispensable for chromatin assembly. However, the remainder of the H3 and H4 histone folds are essential for incorporation of these proteins into chromatin. We suggest that elements of the histone fold domain maintain both nucleosomal integrity and have distinct functions essential for cell viability.

本文言語English
ページ(範囲)12780-12785
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
93
23
DOI
出版ステータスPublished - 1996 11月 12
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 一般

フィンガープリント

「Functional domains for assembly of histones H3 and H4 into the chromatin of Xenopus embryos」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル